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After cultivation overnight at 30°C, the mating mixture was plated onto YPD agar.

The mating mixture was subsequently resuspended in 500  μl of sterile PBS before plating onto selective agar (TYG+erythromycin).

The mating mixture was digested with glusulase and spores were purified using a Percoll gradient as described (Sun et al. 2013).

After 40 minutes of incubation, mating mixtures were plated onto agar containing streptomycin (100 mg/l) and ceftazidime (1 mg/l).

Following matings the controls and mating mixtures were scraped off of the TYG agar plates and re-suspended in 3 ml of sterile 1×PBS, serially diluted and plated in duplicate on TYG agar supplemented with tetracycline and erythromycin for selection of the transconjugants.

Second, after cells were mated on SPA the mating mixtures were resuspended and washed with 1% glucose, rather than H2O.

The timing and level of filamentation in the middle parts of diluted mating mixtures were recorded and compared.

To determine the number of donors and recipients the mating mixtures were also plated onto TYG containing either erythromycin (donors) or tetracycline (recipients).

Mating mixtures were digested with glusulase overnight to eliminate nonspore cells.

Mating mixtures were resuspended in 30 μl LB and deposited onto sterile nitrocellulose filters of 0.45 μm pore size.

The mating mixtures were then plated onto G418, -TRP plates, and a diploid strain was selected from a colony on the plate.