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Furthermore, our work establishes EMS mutagenesis, freezing, and mating methods that allow genetic studies in this species.
The aim was to seek for any structural changes and differences in the mare populations or mating methods that could explain the possible differences between the breeds and years.
A new evolutionary rule was constructed by introducing the simplex-based mutating method and by then combining this with the original cloning and mating methods.
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Screens were performed using a cell-to-cell mating method described previously [ 40].
Thus, this mating method decreased inbreeding and met various demands from breeders.
In general, MC1 seemed to be the preferred mating method, since it almost always yielded the highest genetic response.
The binary vector pBI121-aFGF described above was introduced into A.tumefaciens strain LBA4404 using the triparental mating method.
These results indicated that the random mating method employed in this study was very effective, and the population structure within the RILs essentially did not exist.
The main simultaneous constraints in the mating method were that the relative penalty reductions should be the same for inbreeding and for trait defects.
In this work, we propose a mating method that considers not only inbreeding but also the detailed EBV of progeny or the EBV of sires in reference to acceptance thresholds.
Natural cover - which is the only allowed mating method for the TB - results in very high rates of pregnancy and foaling in the TB industry [ 9- 12], but in other breeds where the AI is allowed and used, higher pregnancy rates have usually been reported for the AI than for the natural mating.
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