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The resulting vectors pRH001-ptsP and -ptsO, -ptsOH30A and -ptsOS61A (Table S1) were transferred by mating into the ΔptsP or ΔptsO mutants to generate the complemented strains ΔptsP/ptsP, ΔptsO/ptsO, ΔptsO//ptsOH30A and ΔptsO/ptsOS61A.
A nocturnal, insectivorous bat, Eptesicus fuscus females separate after mating into maternity colonies that are frequently found in attics of buildings or other manmade locations, since they prefer warmer temperatures in which to raise their young.
Eggs laid in the first six days following mating (into two vials per female) were counted under a binocular microscope.
This clone was transferred using three-way mating into the Agrobacterium strain ASE and transformed into wild type Arabidopsis (Ler) plants.
As with modeled MS-based resistance management, refugia therefore act as a source of susceptible alleles which introgress (via mating) into the pest population, reducing the frequency of Bt-resistant homozygotes.
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Plasmids were mated into recipient Methylobacterium strains using triparental matings as described previously (Fulton et al., 1984).
V-line does were divided according to their ability to mate into group 1 "receptive does" (high sexual desire) and group 2 "nonreceptive does" (low sexual desire) to identify DNA markers useful for association studies with the sexual desire behavior.
The resulting plasmid pJTW019 was mated into PAO1ΔfimL::CTXPexoT-lacZ.
The resulting CTX-PexoT-lacZ plasmid was mated into wild type PAO1 to generate PAO1 CTXPexoT-lacZZ.
The resulting constructs were sequenced at the University of Florida Biotechnology Core facilities, and then mated into S. enterica sv Typhimurium JS246.
Male CB4856 worms were mated into the 23 mutant strains.
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