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Mate preferences, and indeed mating behaviour, are strongly influenced by morphological traits exhibited by a potential partner [1], [2].
When occurring in sympatry with the pipiens form in surface habitats, autogeny and a more generalist mating behaviour are likely to result in a greater fitness molestus form.
As expected from the results concerning gonad specific genes (see above), we found that genes associated with mating behaviour are significantly enriched (p < 0.002) in the age downregulated set.
Rather, field studies in Mali demonstrated strong assortative mating between molecular forms [ 11], which suggests that differences in mate recognition mechanisms, courtship or mating behaviour are probably involved in reproductive isolation, as indicated also by gene expression analysis of various developmental stages [ 92].
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In the bumblebee Bombus terrestris (with CSD), mating behaviour is also affected by relatedness of partners [40].
Male mating behaviour was also strongly age dependent (Fig. 2).
Male mating behaviour was well timed to female ovulation, suggesting that males had accurate information about this.
Male mating behaviour was observed every 30 min within the first 6 h of each day after a set of four virgin females had been added to the mating cage.
To check whether mating behaviour was associated with the survival of adult moths for three days, we also recorded adult mortality daily for three days following the mate choice experiments.
Furthermore, compared with the dioecious species Caenorhabditis remanei, mating behaviour was severely compromised in the N2 strain, i.e. males often fail to find hermaphrodite mates, possibly due to limited production and/or degeneracy of the hermaphrodite's sex pheromone [[ 7, 12] but see, [ 13, 14]].
In no-choice trials, various aspects of mating behaviour were observed and recorded, and for all experiments, an index of sexual isolation was calculated based on the relative numbers of matings between flies in different pair combinations using the program JMating [ 57, 58].
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