Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
Images showing colony formation on SD/MSG + G418 plates in the mating assay are shown.
Similar(59)
To verify the mating type and examine the mating abilities of the derivative strains, a mating assay was performed with the auxotrophic and G418-resistant laboratory haploid strains HR42-11T (α-type) and MCF4741 (a-type) (Fukuda et al. 2013b) (Table 1).
Mating assay was performed as described previously [25], [29].
The yeast two-hybrid mating assay was carried out as described in material and methods.
Under the above conditions, the detection sensitivity of the mating assay was >1 × 10 transconjugants/recipients.
Mating assays are typically performed in agar plates, where hermaphrodites and males are placed in a small spot of bacteria for a few hours or days [ 65, 29, 32, 68].
Mating assays were performed on V8 medium at pH = 5 and pH = 7 [20] and MS medium, pH = 4 [21].
Mating assays were performed on SOE plates (1% sucrose, 0.5% dextrose, 0.5% fructose, 0.1% yeast extract, 0.15% peptone, 2% agar) [30].
Mating assays were carried out on V8 medium [69] (5% [vol/vol] V8 juice, 2 mM KH2PO4, 4% [wt/vol] agar).
Using a Tukey's Honestly Significant Difference test, we found that the outcrossing rate of S. cerevisiae in mass mating assays was significantly greater than all other assay and species combinations.
Mass mating assays were performed once for each of all the pair-wise combinations of the twelve heterothallic strains, as well as for all combinations of the five homothallic S. cerevisiae strains.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com