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Evaluation of mating ability was performed by cultivating yeast cells with a mating partner in 1 ml YPD medium at 30 °C for 1.5 h.
Male mating ability was calculated by dividing the number of male progeny by the total number of progeny (males + hermaphrodites).
We exposed cat-2 lf) cat-2 lfles to exogenous Dadult at least 3 hr and asked if malesg abilito was rexogenous
Male mating ability was investigated using the Bok-rol sy762) Bok-rol sy762 because this mutant istrainile and has a clear roller phenotype.
The embryo mortality was calculated by dividing the number of hatched worms by the number of eggs, and subtracting this ratio from 1. Male mating ability was assayed by placing three males along with one J4 stage virgin hermaphrodite in a 1.5 cm E. coli OP50 lawn on an agar plate.
This decreased mating ability was not due to sluggish movement or paralysis, as they still commenced mating in a similar, but slightly longer timeframe and their backing velocity along the hermaphrodite remained the same (171 ± 43 µm/s n = 6 control, 178 ± 35 µm/s n = 5 25 mM DA).
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Generally, mating ability is repressed in diploid cells due to the presence of both mating-type alleles, but a diploid bearing only one copy of chromosome III can express the haploid mating programme, becoming a so-called 'diploid mater'.
Differences in mating ability were also observed in other similar or identical genotype isolates (B4496 and E296 (high fertility) vs. PAT12ISO1 and E310 (low fertility)) (Figure S2).
C. elegans males, however, have not lost the ability to detect and seek out females from related species like C. remanei that do release attractive pheromones [ 36, 40], although C. elegans male mating ability is poorer than that of C. remanei males [ 35].
Successful escape from predators, foraging and mating ability may be strongly dependant on individual's capacity to accelerate or to achieve a certain speed (Tsuji et al. 1989; Clobert et al. 2000; Vanhooydonck and Van Damme 2003).
We further expect that such mechanism should not lead to a reduction in mating ability when there is no variation in mate size.
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