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If present, this could explain the high transformation efficiency, natural competence, and mating ability of Hfx.
However, unlike wild-type males, the mating ability of sir-2.1 0) sir-2.1 0ly droprematurely.
In all cases, reference strain PD4790 was the strain that failed to mate successfully, which corroborates the relatively poor male mating ability of the N2 genetic background [ 29, 30].
Although males with mutations in both K+ channels abrogate most of the beneficial effects of transient starvation, we still observed a small increase in the mating ability of aged double-mutant males, suggesting that other mechanisms are applied by transient starvation to improve mating (Guo et al., 2012).
This behavior cannot be due simply to greater mating availability or mating ability of more viable cells, because less viable focal cells mate as efficiently as more viable focal cells, and the mating advantage of more viable sized cells is not affected by whether the focal cell is large or small.
For example, it would have been possible for increased mating ability of males to have countered increased resistance to mating by hermaphrodites.
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Fig. 4 Evaluation of mating abilities of the derivative strains K76A and K76AL.
We implemented the PC analysis in JMP, including values for the following traits for each of the 7 strains (Table 2): courtship ability, mating ability, number of sperm transferred, spermatid size, sperm production rate, P2-early and P2-late.
We show that, compared to the original population, colony males but not females have increased their insemination rates in the laboratory in both types of mating, suggesting that faster-male evolution of mating ability might have occurred during the colonization process.
According to theoretical models, high inbreeding depression, high male mating ability, and high a rate of production of males by hermaphrodite self-fertilization are factors required for maintenance of androdioecy in C. elegans [ 18].
Evaluation of mating ability was performed by cultivating yeast cells with a mating partner in 1 ml YPD medium at 30 °C for 1.5 h.
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