Briefly, C57BL/6 males were injected with ENU and outcrossed to C3H/HeH; male F1 offspring were mated to C3H/HeH and F2 females backcrossed to their father.
Therefore, we carried out another experiment in which engorged, mated females were injected with 1 µl PBS/10% DMSO (n = 12) or 1 µl PBS/10% DMSO with 10 µg rifampin (n = 14).
Time-mated females were injected intra-peritoneally with 500 μg/kg dexamethasone or vehicle (saline) at E14.5 then culled 6 h post-injection.
Mated female flies were injected with 10 ng/μl of blt dsRNA, control dsRNA, or with buffer and individually placed for 24 h into small glass tubes with food.
For CX3CL1 treatment, homozygously mated BALB/c females were injected intraperitoneally with 1 μg of CX3CL1 (R&D Systems, Minneapolis, MN, USA) or placebo (PBS containing 0.1% BSA) on GD6.
For IFN- γ treatment, homozygously mated BALB/c females were injected intraperitoneally with 5000 U IFN- γ (Peprotech, London, UK) or placebo (sodium phosphate containing 0.1% BSA) on GD6.
Mice were given BrdU (50 μg/g body weight) by i.p. injection according to the following schedule: Time mated (E18) pregnant Gdf15 +/- females were injected once and sacrificed after 2 hours or they were given a second injection 3 hours after the first one and sacrificed after 3 further hours.
IL-4 receptor alpha−/− females were mated to wild-type males, and pregnant females were injected with supraphysiologic doses of IL-4 or 13.
Though the sad fact is that it still has a way to play yet, and will not be over until Watson and his 33 team-mates know once and for all exactly what they were injected with.
