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Symmetry mates are generated on the mobile device's GPU by multiplying OpenGL's model-view matrix with symmetry operators.
Hybrid mdx/Y∶R26/+ males were generated by mating R26 males with C57BL/10ScSn-mdx/J C57BL/10ScSn-mdx/Jing femalesgeny candying both the R26 allele and mdx mutation.
To see what other pair relationships were worth considering, we simulated 100,000 lines such that random sib-matings were used for 15 generations, at which time three mating pairs were generated, producing two male and two female offspring each.
Mated females were generated and maintained as for lifespans.
Multiple sequence alignments of the selected shotgun sequences and their mate pairs were generated by the sequence assembly script PhredPhrap [ 21- 23].
Mated populations were generated by mating young hermaphrodites with young males (1 6 ratio of hermaphrodites to males).
Mate pair libraries were generated using the Nextera Mate Pair protocol (Illumina, San Diego, CA).
Mate pair libraries were generated as per the SOLiD Mate Pair Library preparation protocol.
Long insert mate-pair DNA libraries (size range from ca. 2 kbp to 20 kbp) Eight mate-pair libraries were generated for the emperor penguin, and five mate-pair libraries were constructed for the Adélie penguin (Additional file 1 : Table S1).
Eight mate-pair libraries were generated for the emperor penguin, and five mate-pair libraries were constructed for the Adélie penguin (Additional file 1: Table S1).
In total twenty-eight long read runs, six runs on 2.5 kbp mate-pair libraries, and six runs on 5 kbp mate-pair libraries were generated.
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