Sentence examples for maternal pronucleus from inspiring English sources
After a spermatozoon fertilizes the oocyte, the protamines on the sperm chromatin are replaced by histones (van Meel and Pearson, 1979) before paternal pronucleus (PPN) and maternal pronucleus (MPN) formation.
The establishment of polarity is completed before the maternal pronucleus starts to migrate to the posterior, and the hallmark of completed establishment phase is pseudocleavage furrow formation [10], [15], [19].
Meanwhile, the maternal pronucleus becomes widely methylated.
The relative amount of paternal methylation was calculated as the ratio of paternal to maternal pronucleus integrated fluorescence signal densities.
This corresponded to a strong 5-mC signal in the maternal pronucleus and its loss in the paternal pronucleus.
The 5-hmC was firstly detected by immunofluorescence staining at both paternal and maternal pronuclei in the PN1 zygote.
The new mei-1 mutants disrupt female meiotic spindle function and produce one-cell stage embryos with large misshapen polar bodies and a variable number of maternal pronuclei (range = 0 9; see Fig. 5A, B).
Only zygotes with minimal or no overlap between paternal and maternal pronuclei were used for the analysis.
We have recently shown that the amount of 5hmC also increases in the maternal pronuclei, whereas the level of 5mC decreases [ 19].
After the background subtraction the areas occupied by paternal or maternal pronuclei were defined manually and the integrated fluorescence signal densities (pixels) were calculated by the software.
