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Imprinted genes play a critical role both in prenatal and postnatal growth, the latter being mainly influenced by the maternal performance of the dam.
To summarize, differential expression patterns of candidate genes in a number of pathways, including tight junction, MAPK and Wnt signalling pathways were associated with enhanced maternal performance of QSi5 mice relative to CBA mice.
To determine whether the increased maternal performance of QSi5 mice was independent of litter size we adjusted litter sizes on day of parturition for both the QSi5 and CBA strains, and calculated cumulative weights for a standardized six pup litter.
These results confirm that the superior maternal performance of the QSi5 mice was independent of the large litter size phenotype, and that significant differences in maternal performance exist between the QSi5 and CBA inbred strains of mice.
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Thus, differential expression of components of the tight junction, Wnt and MAPK signalling pathways together with favourable genomic imprinting may contribute to the increased maternal performance phenotype of the QSi5 strain of mice.
To identify the genes associated with the enhanced maternal performance phenotype of the QSi5 strain, gene expression profiles of mammary gland tissue from five animals each of lactating QSi5 and CBA mice were compared.
In the present study, a comparative analysis of gene expression in QSi5 and CBA mice identified candidate genes in a number of pathways, including tight junction, MAPK and Wnt signalling pathways, as contributors to the superior maternal performance phenotype of the QSi5 strain.
Importantly, even when previously neglectful mice do raise litters (via cross-fostering), they negatively affect offspring's fitness as adults as indicated by decreased maternal performance and increased expression of risk factors for maternal neglect.
As with all maternal performance traits, the unit of analysis was the lamb, rather than the mother: thus twin lambs both received a separate data record.
Hence, a relatively lower level of Grb10 and Igf2r gene expression is consistent enhanced maternal performance.
Comparison of the two phenotypically divergent inbred mouse strains, QSi5 and CBA, identified genes associated with maternal performance.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com