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Materials were incubated fully water saturated at 15°C for 70 days; peat-only and litter incubations served as controls to the full-factorial re-combination of peat and litter.
Electrospun scaffold strips of these materials were incubated with solutions containing enzymes that catalyze hydrolysis, or solutions containing oxidative species.
The efficiency of sterilization was confirmed after randomly chosen samples of model materials were incubated on Potato Dextrose Agar (PDA, BTL, Poland) at 28 °C.
Fresh normal breast tissue and breast tumor materials were incubated for 1 hour at room temperature in tissue mix consisting of DMEM/F12, 1% fungizone, 1000 U/ml penicillin, 1000 µg/ml streptomycin, 10 µg/ml insulin and 10% bovine serum (all from Invitrogen).
Primary antibodies (Table S3, Supplemental Materials) were incubated with the membranes and recognized with horseradish peroxidase conjugated secondary antibodies.
These materials were incubated with clinical isolates of S. aureus and P. aeruginosa, listed in Table SI2 in the Supporting Information.
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Each set of glass jars (10 cm × 10 cm) containing mixtures of soil and organic materials was incubated for 10, 20, and 30 days in an oven.
In this process, the plant materials are incubated at optimum conditions for the activity of the heterologously expressed enzymes, then a pretreatment is performed and subsequently the materials are saccharified with exogenous enzyme.
Human digestion models can first be simple and static, and mimic only gastric or small intestinal conditions, in which materials are incubated with simulated gastric fluids, simulated small intestinal fluids or buffers at static pH values (Mwilu et al. 2013; Minekus et al. 2014).
To lyse the cells, microplates with the sorted material were incubated at 37°C and 75°C, 20 min each.
Therefore, tissue-materials were incubated in 1 m M orthovanadate solution at 200 mmHg for 5 min (Akimoto et al, 1998).
More suggestions(15)
leaflets were incubated
pieces were incubated
contents were incubated
materials were begged
materials were donated
materials was incubated
materials were scrounged
materials were dispersed
materials were extruded
materials were harvested
materials were used
materials were investigated
materials were stolen
materials were weighed
materials were simulated
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