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All the materials were frozen in liquid nitrogen quickly after they were taken from the plant and then stored at –80 °C until use.
All the plant materials were frozen immediately in liquid nitrogen to quench the enzymatic activity.
All materials were frozen in liquid nitrogen immediately after collection for ribonucleic acid (RNA) isolation.
All materials were frozen immediately in liquid nitrogen, and stored at -80°C until RNA isolation.
Plant materials were frozen in liquid nitrogen and ground with IKA A11basic (Staufen, Germany).
Plant materials were frozen in liquid N2, and stored at −80 °C until further use for total RNA isolation.
Similar(52)
Samples of fresh material were frozen at -22°C for later analysis.
Chimney material was frozen at −80°C immediately after collection and remained frozen until onshore analysis.
Below a certain temperature, known as the glass transition temperature (Tg), the molecules of a polymer material are frozen in what is known as the glassy state; there is little or no movement of molecules past one another, and the material is stiff and even brittle.
C-V curves measured at 77 K (Fig. 3b) show much lower hysteresis than those obtained at room temperature, thus indicating that some traps in the material are frozen at low temperatures [16].
Plant material was frozen in liquid nitrogen.
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