Sentence examples for materials were eluted from inspiring English sources

Precipitated materials were eluted with 300 μl elution buffer (1% SDS; 0.1 M NaHCO3; 50 mM Tris HCl, pH 8; and 10 mM EDTA).

After washed with ChIP wash buffer (50 mM HEPES-KOH (pH 7.0), 0.5 M LiCl, 1 mM EDTA, 0.7% deoxycholate, 1% NP-40) five times and with TE (10 mM Tris-HCl (pH 7.5), 1 mM EDTA) once, immunoprecipitated materials were eluted from the beads by heating overnight at 65 °C in elution buffer (50 mM Tris-HCl (pH 8.0), 10 mM EDTA, 1% SDS).

After extensive washing, bound materials were eluted with Laemmli buffer and analysed by immunoblotting.

The concentrated material was applied to the immunoaffinity column at 4°C and the adsorbed materials were eluted with 0.3 M acetic acid containing 0.1% 2-mercaptoethanol.

The bound materials were eluted by a buffer containing FLAG peptide (0.2 mg/ml).

The adsorbed materials were eluted with a citrate-NaOH buffer, pH 4.0, after washing the column with 4 mL of the equilibration buffer.

At this stage, unreacted monomers and other low molecular weight materials are eluted along with low-affinity polymer nanoparticles.

The column was then washed with PBS and the bound material was eluted with elution buffer containing 10 mM Glutathione in 50 mM Tris-HCl, pH8.0.

The immunoprecipitated material was eluted in elution buffer (100 mM NaHCO3, 1% SDS) and was then digested with proteinase K and RNase H to remove protein and RNA, respectively.

After washing, the supernatant was removed and total material was eluted with 100 µl elution buffer (0.1% SDS, 2.5 mM Tris pH 6.8 and 5 mM DTT) at room temperature.

Finally, the remaining material was eluted with 1 wt% DOC, which resulted in complete elution.