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The materials were dehydrated in an acetone series and embedded in the Spurr's resin (Electron Microscope Sciences, Washington, PA).
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After washes in PBS, the material was dehydrated in acetone and embedded in Epon.
After rinsing in 0.1 M phosphate buffer, the material was dehydrated in a graded alcohol series and acetone, and embedded in Epon 812 epoxy resin.
Finally, material was dehydrated through an ethanol series, and embedded in Spurr.
The fixed material was dehydrated in a graded alcohol series and embedded in Epon 812.
All material was dehydrated with ethanol and embedded in Epon 812.
This material was dehydrated in a graded series of ethanol (20% to 100%).
Such prepared material was dehydrated in alcohol-acetone series (30 100%) and embedded in Epon 812 (Sigma Aldrich, Poland).
The fixed material was dehydrated through an ethanol and ter-butanol series and then embedded in paraffin.
After fixation, the material was dehydrated through a graded series of alcohols and embedded in Spurr Resin.
Fixed material was dehydrated through a graded ethanol series and embedded in paraffin wax (Johansen, 1940; Ruzin, 1999).
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