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The electrospun materials were collected with an ethanol bath adapted to a previous electrospinning set-up.
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The deposited material was collected with silicon substrates attached to the hollow airway's walls in different points.
All donor material was collected with the approval of the Scientific Ethics Committee of the Capital Region, Denmark (Protocol H-3 2013 054) H-3 2013 054informed consent wandobtained according to the Declaration of Helsinki.
Immunoprecipitated material was collected with protein A agarose beads (Millipore) and washed sequentially with a low salt-immune complex wash buffer (Millipore), a high salt-immune complex wash buffer (Millipore), LiCl Immune Complex Wash Buffer (Millipore) and 1X TE (10 mM Tris-HCl, pH 8.0, 1 mM EDTA) for 5 min on a rotator at 4°C.
All the biopsy material was collected with necessary ethical clearances and after obtaining informed consents.
For patient 2, decreased concentrations of MMP1, -2, -3, and -7 were observed when sample material was collected with flocked swabs as opposed to aspiration.
The material was collected with a license from the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA, license 032/2005-CGFAU/LIC).
The cervical and endocervical material was collected with the aid of an Ayre spatula and a cytobrush for cervical smears and for PCR amplification (suspended in 1 mL of 0.9% NaCl solution and stored at −20°C until analysis).
Material was collected with full ethics approval from a local ethics committee (MREC/01/4/061 and LREC 02/281 M) and as part of the ProMPT Study: Molecular mechanisms and the development of novel treatment strategies in progressing prostate cancer – Northern (and Bristol Prostate Cancer Collaborativee).
Then, the materials were collected and cleaned with in sterilized water.
Solid materials were collected by filtration, washed with dried toluene, ethanol reflux for 24 h and then dried at 70 °C overnight.
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