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In brief, calU16 was PCR amplified from genomic DNA and cloned into NdeI/XhoI-digested pET15 expression vector (NESG Clone ID MiR12 15.1; PSI Biology Materials Repository clone ID MeCD000597015, see http://psimr.asu.edu/) to encode for the corresponding N-terminally tagged (MGHHHHHHSHM) fusion protein.
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Materials repositories include the Jackson Laboratory, the European Mouse Mutant Archive (EMMA), the European Conditional Mouse Mutagenesis Program (EUCOMM), the Knockout Mouse Project (KOMP), Addgene, RIKEN Bioresource Centre, the Mutant Mouse Regional Resource Centers, American Type Culture Collection (Americas), American Type Culture Collection (Asia/Europe), and the UK Stem Cell Bank.
Vectors pCMVi-UB, pCMVi-LSrCOMPTT, pCMVi-LTA (also called pCMVi-LS-LTA-R192G) and pCMVi-LTB were available from the PSI Biology-Materials RePSI Biology-Materials.oRepositoryzona State University.
Moore secured funding from the National Institutes of Health (NIH) and applied for samples of the different serotypes from the Centers for Disease Control and the American Type Culture Collection, a biological material repository headquartered in Virginia.
The first three years a traditional strategy was followed and a course management system was used as material repository.
In the course of her book, she explores five interrelated "spaces" where electronics fall apart: from Silicon Valley to Nasdaq, from containers bound for China to museums and archives that preserve obsolete electronics as cultural artifacts, to the landfill as material repository.
Seiler, C. Y. et al. DNASU plasmid and PSI Biology-Materials rePSI Biology-Materials to accelerepositoriescal resources
Curtarolo, S. et al. AFLOWLIB.ORG: a distributed materials properties repository from high-throughput ab initio calculations.
Curtarolo, S. et al. AFLOWLIB.ORG: A distributed materials properties repository from high-throughput ab initio calculations.
This effort was made possible by combining and building upon a number of key components, such as the CALPHAD journal's supplementary information section, the NIMS database, the NIST materials data repository, the Crossref bibliographic service, and various thermodynamic (OpenCalphad, ATAT) and graphical (gnuplot, XTK) software.
To this end, an Application Program Interface (API) following REST principles is introduced for the AFLOWLIB.org materials data repositories consortium.
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