It has to be noted that the tested E. globoidea was genetically unimproved material of unknown provenance.
Because these characteristics are very insignificant and show a high individual variability, most of these forms were probably not true subspecies, especially as they were often based upon zoo material of unknown origin that may have had "striking, but abnormal" morphological characteristics.
Unbalanced chromosomal abnormalities (N = 80) detected included: 18 numeric changes of whole chromosomes; 22 chromosomal deletions and five duplications; nine with additional chromosomal material of unknown origin; 17 with derivative chromosomes and three with marker chromosomes.
CMA further identified the additional material of unknown origin and delineated breakpoints on all derivative chromosomes, thereby often having the added advantage of refining genomic intervals involved in the imbalance (N = 26) such as in case V4-31, case V5-41 and case V5-35 (Table S1 and S2).
PCRs were carried out in 25 µL volumes: 18.25 µL water, 2.5 µL 10X buffer (New England Biolabs), 1 µL (400 µM) dNTPs, 0.25 µL Taq polymerase (New England Biolabs #M0267L), 1 µL (0.8 µM) per primer, and 1 µL (40 ng) of purified Vigna mitochondrial DNA (from cv. Berken) or 2 µL (30 ng) of total Vigna DNA (from material of unknown genetic ancestry purchased at local grocery store).
However, the activated microglia in Plp1tg mice often contain degenerating material of unknown origin.
We assume that some strain-specific genetic material of unknown function is associated with the development of endofungal lifestyle.
Concurrent chromosome analysis for case 55 revealed a chromosome 17 with additional material of unknown origin in 25% (5/20) of cells.
Indeed, our EM analysis shows that some secretory material (of unknown identity) remains in the SSCs, even once Otogelin-like is knocked down (Fig. 8C).
Case 107 (also a mixed endometrioid and mucinous carcinoma) showed additional material of unknown origin on 8q24 as the sole chromosomal abnormality.
