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Our results have implications for the design of new materials for soft tissue engineering, where native tissues have moduli in the kPa range.
Artificial composites designed to mimic the structure and properties of native extracellular matrix may lead to acellular materials for soft tissue repair and replacement, which display mechanical strength, stiffness, and resilience resembling native tissue.
A wide variety of biodegradable polymers is required, ranging from pliable, elastic materials for soft tissue regeneration to stiff materials that can be used in load-bearing tissues such as bone in order to engineer scaffolds suitable for different applications.
Hydrogels are widely used as biomaterials in biomedicalfields such as in drug delivery systems, cell-based therapies, wound dressing, implant materials for soft tissue and anti-adhesion materials due to its unique characteristics.
Despite their drawbacks, most clinically used materials for soft tissue repair are based on synthetic polymers such as polypropylene and silicones or collagens from allogeneic and xenogeneic sources.
Collagen (Col) and gelatin (Gel) were selected as the main components for 3D scaffolds since, in many senses; they are ideal materials for soft tissue engineering constructs.
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Present work in our laboratory seeks to address the challenge of developing biocompatible adhesive hydrogel materials for soft-tissue adhesion and repair.
Hydrogels represent to be an interesting starting material for soft, and lately also for hard tissue regeneration.
Polydimethylsiloxane (PDMS) is the most popular and versatile material for soft lithography due to its flexibility and easy fabrication by molding process.
Overall, this study shows the efficacy of SC-CO2 method for delipidation and decellularization of adipose tissue whilst retaining its ECM and its subsequent utilization as a bioactive surface coating material for soft tissue engineering, angiogenesis and wound healing applications.
Preadipocytes, immature precursor cells found between mature adipocytes in adipose tissue, are a potential material for soft tissue engineering since they can proliferate and differentiate into adipose tissue after transplantation.
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