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BAC libraries from four rice varieties and 14 wild rice accessions were used as primary materials for sequencing the whole genomic regions of the Hd6 gene (Table 1).
DM created the oadb website, designed and instantiated the GitHub repository and wrote the commentary, AE and AD sourced biological materials for sequencing, DGOS, KY and SK prepared biological materials, managed sequencing and performed analyses and contributed data to the repository.
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In other cases, researchers create genomic or complementary DNA (cDNA) libraries to identify interesting genes or to provide cloned material for sequencing.
Five obese, hyperleptinemic and five obese, nonhyperleptinemic mares were selected to study the possibility of polymorphism in exon 2. First, in experiment 2, forward and reverse primers were designed from the Bos taurus leptin gene (GenBank Accession # U50365) to identify and subsequently clone the equine leptin gene to provide material for sequencing.
CC collected and processed finch material for sequencing.
Once DNA is extracted, small yields require amplification to obtain enough material for sequencing.
This final point is particularly relevant to researchers who are considering the use of WGA amplified material for sequencing.
In most cases, we had to perform a nested PCR reaction to get sufficient material for sequencing.
The density of linear peptide inside the bead was kept high to provide enough material for sequencing.
Prior to sequencing, oocysts were isolated directly from field specimens without propagation in laboratory animals, and extracted DNA was amplified to generate enough material for sequencing.
However, the instruments involved still require the selective amplification of target DNA and the relatively complex preparation of this material for sequencing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com