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The development of highly functionalized porous materials for protein separation is important for biotech processes.
To test these materials for protein separation, bovine serum solution was added in the sample.
Aminoacyl-tRNA synthetase (aaRS) serves to generate the raw materials for protein synthesis aminoacyl-tRNA in organisynthesis aminoacyl-tRNA
To use these materials for protein separation, the sample was washed several times to make sure there is no free gold nanoparticle in the solution, and bovine serum solution was added in the sample.
Materials for protein purification and SDS-PAGE were purchased from GE Healthcare and Bio-Rad.
Pyrolytic graphite, graphene, glassy carbon, as well as conductive diamond electrodes can be considered as convenient electrode materials for protein analysis.
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Polystyrene is a common substrate material for protein adsorption in biosensors and bioassays.
We describe the microfluidic production of cell-mimics with a porous polymer membrane containing an artificial hydrogel compartment, which resembles a eukaryotic cell's nucleus in that it contains the cell-mimics' genetic material for protein synthesis and can sequester transcription factors.
The developed method conditions were specific, accurate, and sensitive for detecting bis-mal-PEG as an impurity in mono-mal-PEG with limit of quantitation of 0.2% and may be used to assess the quality of mono-mal-PEG as a raw material for protein PEGylation.
Kim et al. [78] also demonstrated that graphene-coated surfaces are potentially valuable as conducive material for protein attachment due to the presence of hydrophobic and hydrophilic patches.
This suggests a high sensitivity of the electrode for protein recognition even though no mediator is incorporated, which also implies the label free capability of the electrode material for protein detection.
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