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Cell debris and insoluble material were cleared by centrifugation at 10,000× g for 10 min.
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Potential explanations for negative scans include the following: (1) patients really did not aspirate or they refrained from eating before bedtime and reflux did not occur; (2) patients had reflux, but aspiration was infrequent; and (3) aspiration did occur but the material was cleared during the 8-hour interval.
Most sensitive material is cleared for publication after 30 years, although some remains under wraps even longer.
The insoluble material was cleared by centrifugation at 11,000× g at 4°C for 10 min before 500 µl aliquots of the supernatants were injected onto Superose 6HR column (GE Healthcare) equilibrated with 50 mM Tris-HCl, pH 7.4, 150 mM NaCl and PFO (0.5%) or SDS (0.1%).
After incubation, plant material was cleared with 70%% ethanol.
Insoluble material was cleared by centrifugation at 10 000 g for 10 min.
Insoluble material was cleared with a 30 minute centrifugation (8,000 x g) and the supernatant was retained for protein purification.
Insoluble material was cleared by high-speed centrifugation and the cleared lysate applied to a 5 ml HisTrap column using an AKTA system (GE Healthcare).
Once the material was cleared, it was mounted on a microscope slide with one or two drops of Visikol, and a cover slip was added.
After homogenization, insoluble material was cleared with a 30 minute centrifugation (8,000 x g) and the supernatants were retained for immunoblot analysis.
Insoluble material was cleared by centrifugation at 10,000 × g for 10 minutes and protein was quantitated using bicinchoninic (BCA) (Pierce Biochemicals, Rockford, IL, USA).
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