NMR studies showed that the bioactive material we had isolated from T. parthenium was indeed a mixture of the two epimers 1 and 2 with the major isomer isolated from this plant having the same relative stereochemistry as 2 [compare Figure 2a and c (full line)].
As described in Materials and Methods, we isolated three independent clones from each of five FGF1-specific shRNA plasmids and compared FGF1 expression in all 15 cell lines by qRT PCR analysis (data not shown).
Using another aliquot of the section material for SDS-PAGE, we isolated single bands of expected weight, as already described for the microdissected cells.
Guided by fate maps (Garcia-Lopez et al., 2009) and optic vesicle morphology (supplementary material Fig. S3), we isolated optic vesicle (OV) explants from stage 9, 10, 11 and 13 embryos.
This analysis was performed for four of five patients with sIBM because of lack of biopsy material from patient 4. We isolated AIBM, NIBM, and HCTRL myofibers from five patients and three control individuals, as described in Materials and Methods and illustrated in Figure 1.
As a result of the source material, we see only isolated inklings of Hawking's genius.
To isolate any virus material, we injected small amounts of the blood samples into VERO cells, an easily replicable cell lineage that is used a lot in labs.
To identify haustoria enriched transcripts, we compared gene expression levels between infected material and isolated haustoria.
Attempts to define kazari -- to disassemble its mechanism, isolate its parts for study -- has given way to a free-for-all of material muchness, isolated technical virtuosity, trash-with-flash glamour.
Cell wall material was isolated from the endosperm of the mono- and dicotyledons.
