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Tissue material was vortexed and trypsin (1.25 mg/mL, BioChrom, Berlin, Germany) and EDTA (0.01%, Ethylenediaminetetraacetic acid, Sigma-Aldrich) added and the tissue was again triturated 15× and incubated rotating 20 min at 37°C, 5% CO2.
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The material was vortex for 1 min and then placed on ice for 1 min; the procedure was repeated 10 times.
After adding the two reference materials together, it was vortexed and mixed overnight before DNA isolation.
The tube was vortexed to remove all the material from the swab, which was discarded.
The mixture was vortexed for 60 s.
The mixture was vortexed vigorously.
The dilution was vortexed.
Mixture was vortexed and centrifuged.
Reaction mixture was vortexed for 15 20 s.
The suspension was vortexed and filtered.
The mixture was vortexed for 10 seconds.
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