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Western blots were performed with a mouse anti-ABri polyclonal Ab [19] (1∶1,000 dilution) The secondary antibody (1∶2,000 dilution) was peroxidase-linked anti-rabbit IgG (Amersham Biosciences, Piscataway, NJ), and the immunoreactive material was visualized as chemoluminescence on X-ray film with an ECL detection kit (Pierce).

Immunoreactive material was visualized by chemiluminescence (ECL, Western Blotting Detection, GE Healthcare, Madrid, Spain) according to the manufacturer's instructions.

The material was visualized using a Zeiss laser scanning inverted microscope (Axiovert LSM510) (Carl Zeiss MicroImaging, Inc., Thornwood, NY, USA).

Immunoreactive material was visualized using the Immun-Star WesternC Kit (Bio-Rad, Hercules, CA, USA) products and bands were detected via exposure to film (Kodak, Japan).

TEM gave a more detailed picture of the exudation, and material was visualized emitting from the flagellar pocket, at the hemidesmosome (Fig. 8D and E) and apparently budding from the flagellum itself (Fig. 8F H).

After washing, blots were incubated for 1 h with peroxidase-conjugated antibody as a secondary antibody, prepared at a 1 5,000 dilution (Molecular Probes Inc .. Immunoreactive material was visualized by chemiluminescence (ECL, Western Blotting Detection; GE Healthcare, Madrid, Spain) according to the manufacturer's instructions.

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The grafted material is visualized as a low density monomolecular layer of covalently bonded material.

The spatial distribution of soil materials was visualized using a three-dimensional soil layer model.

Immunoreactive materials were visualized using a biotinylated anti- rabbit IgG antibody (Dako), peroxidase-labeled streptavidin, and diaminobenzidine.

The radiopaque materials were visualized without problems during injection in 208 augmentation sites.

For more information and photosensitized formation of a superoxide anion, see Supplementary Material 2. The chicken CAM vasculature was visualized and irradiated with light for the photoactivation of sunitinib using an epi-fluorescence microscope (Nikon AG, Eclipse E 600 FN, Tokyo, Japan) with objectives Plan Apo × 4/0.2 or Plan Fluor × 10/0.3 (Nikon AG).

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