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The ground plant material was successively extracted withPetrol, EtOAc, MeOH, and water in a Soxhlet extractor.
Plant material was successively extracted with 5 volumes (v/w) of n-hexane, chloroform, chloroform : methanol 9 : 1, and methanol by a sequential maceration, as described previously [ 11], obtaining 4 extracts with increasing polarity: n-hexane (HRE), CHCl3 (CRE), CHCl3 : MeOH (9 : 1) (CMRE), and MeOH (MRE) root extracts.
The plant material was successively extracted in the same manner with solvents of rising polarity to obtain a dichloromethane extract (1.4 g), an ethyl-acetate extract (0.7 g), a n-butanol extract (5.4 g) and finally a methanol extract (12.4 g).
Similar(57)
A buildup process in which a large number of thin layers of additional material are successively attached to the interior cylindrical surface of the arch is considered.
In the present work, coated and non-coated tin pyrophosphate materials were successively prepared and their electrochemical performance were compared.
The QC1 and QC2 materials were successively analyzed daily over a 20-month period.
The plant materials were successively extracted with hexanes, dichloromethane, and methanol (2 × 100 ml for 24 h each).
25 g of ground plant material was extracted successively with distilled H2O in Soxhelt extraction apparatus.
In order to avoid genetic variance due to a lack of isogenic lines from this non-model organism that barely self-pollinates, all petal material was harvested successively from one individual plant.
The dried and powdered plant material (leaves, 1000 g) was successively soaked twice during 48 h in n-hexane, dichloromethane, ethyl acetate and methanol at room temperature (3 L x 2 each).
This technique reproduces putative key steps of prion replication in an accelerated manner in the test tube, and demonstrated the auto-seeding activity of newly generated PrP aggregates in multiple serial passages (i.e., after the initial seeding material had been successively diluted out).
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