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Many features of the two designs had qualitative similarities but for levels of fill exceeding 60% the material was stirred essentially as a solid body by the six-bladed agitator.
About 100 g of the powdered plant material was stirred with 500 ml distilled water at 95 °C for 25 min.
Half gram of the powdered root material was stirred in 5 ml of 1% aqueous hydrochloric acid on a water bath and filtered.
This material was stirred in deionized water until the mixture became homogeneous, filtered through a Miracloth and stored at 4°C in airtight plastic bags until it was used in the enzymatic hydrolysis experiments.
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Once these materials were stirred slowly, the extent of Resazurin reduction was determined through the extent of absorption at the wavelength of 610 nm using UV/Vis spectrophotometer device.
Afterwards these materials were stirred slowly, The rate of alamar blue reduction was determined through the extent of absorption at the wavelength of 620 nm using UV/ViS spectrophotometer device (Braic 2100) (Toolabi et al. 2017; Zare et al. 2016).The percentage of alamar blue reduction was obtained using Eq. 3.
TLC still revealed presence of the starting materials and the mixture was stirred for further 8 h at room temperature.
The starting material dissolved and the reaction was stirred at room temperature for 3 h.
The resulting mixture was stirred at room temperature until the starting material was vanished by TLC check.
The resulting mixture was stirred at 0 °C until disappearance of the starting material was observed by TLC (about 1.5 h).
15 g of the given powdered plant material was mixed in 75 mL of each solvent and the mixture was stirred for 24 hours.
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