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Suspensions were kept on ice for 60 min, insoluble material was spun down in a microfuge (3 min, 13.000 rpm, 4°C) and the supernatant transferred into fresh tubes.

Following lysis, insoluble material was spun down at 5600 g for 20 min at 4°C.

After the overnight incubation, undigested material was spun out and the Maxwell cartridges were loaded.

The precleared material was spun at 11000 g for 15 minutes and the supernatant was transferred to a fresh tube.

A 13.5 μL of 10% (wt/vol) digitonin (Calbiochem, Billerica Massachusetts U.S.A). in dimethylsulfoxide (DMSO) was added and gently mixed on ice for 3 min. This suspension material was spun at 22,000 g for 8 min. The mitochondrial pellet was then resuspended to a final volume of 1.5 mL of MS buffer plus BSA and centrifuged at 22,000 g for 10 min at 4°C in a microfuge.

Similar(55)

These precursor materials were spun and then processed into carbon fibers.

RNA was extracted by hot acidic phenol/chloroform treatment [70] from 0.2 ml of the upper layer ( =  fraction enriched for cytoplasmic material) and 0.1 ml of the material that was spun down to the bottom of the tubes (enriched for nuclear material) and suspended in 0.3 ml of buffer P containing 0.45M sucrose.

Thirdly, PCBM material is spin-coated on the top of P3HT nanogratings, and the electrode is deposited onto the PCBM surface, to complete the fabrication process of OBHJ solar cell.

Mr. Heyman was most recently chairman of the GAF Corporation, a manufacturer of roofing materials, and a chemical company that was spun off from GAF, called International Specialty Products.

It may not look like a bunch of cobwebs, but the tie you see above was spun from the same material spiders spin out from their behinds.

In 2004, Nextreme was spun off of RTI to develop a thermoelectric material for semiconductors commercially.

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