Sentence examples for material was solubilized from inspiring English sources

Following this washing step the material was solubilized in 500 µl of urea lysis buffer (7.5 M Urea, 2.5 M Thiourea, 40 mM Tris pH 7.6, 2.5% Octyl-b-glucoside, 6.25 mM TCEP, 1.25× Proteinase inhibitor) followed by homogenization on ice 10 times using a Potter homogenizer.

Insoluble material was solubilized by vigorous agitation in 6 M GuHCl, re-homogenized and left for four hours at room temperature (RT) before storage at −80°C.

After sonication, DDM was added to 0.5%, and lysed material was solubilized for 1 h on a rotary platform in a cold room (4 °C).

Insoluble material was solubilized by vigorous agitation in 6 M GuHCl, re-homogenized and left for 4 h at room temperature (RT) before storage at −80°C.Total protein concentrations were determined using the Total Protein Kit (Sigma Aldrich, Dorset, UK) following the manufacturer's guidelines.

Wells were washed 3 times with ice-cold 96% ethanol, residual cell material was solubilized with (1% SDS, 2%Na2CO33, 0,1 M NaOH) and radioactivity was measured by scintillation counting.

TBS-soluble materials were solubilized in Laemmli sample buffer and subjected to SDS-PAGE.

The resulting lysates were clarified by centrifugation (3200 x  g), and after removal of the soluble material the insoluble pellet was solubilized with 4 M urea.

That is, 86% of all xylan was solubilized but, as not all liquid phase is pressed out, some solubilized xylose remains in the liquid phase of the pressed material.

The starch was solubilized by autoclaving.

SIL was solubilized in PBS.

The final precipitate was solubilized in water and lyophilized.