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The debulked material was snap frozen in liquid nitrogen and then stored at −80°.
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In brief, tumour material was snap-frozen in liquid nitrogen within 1 h after surgery.
The tumour and metastatic material was snap-frozen in liquid nitrogen within 1 h after surgery.
Tumor material was snap-frozen in liquid nitrogen immediately after surgery.
Tumor material was snap-frozen in liquid nitrogen directly after surgery.
For total leaf extracts, 250 mg fresh material was snap-frozen in liquid nitrogen and then ground in a ball mill (Retsch, Haan, Germany).
The material was snap-frozen and tissue blocks were positioned in a cryostat to allow cutting sections in a longitudinal plane of the vessel encompassing the entire thickness of the vascular wall.
The explants, which included the tissues surrounding the implanted material, were snap frozen in a dry ice/acetone mixture.
A total of 0.5 g materials was snap-frozen in liquid nitrogen and stored at −80°C.
Upon harvesting, all plant materials were snap-frozen in liquid nitrogen and ground into a fine powder using an IKA A11 grinder cooled with liquid nitrogen.
The taproot material that was snap frozen and stored at −80 °C was ground to a fine powder in liquid nitrogen with a mortar and pestle.
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CEO of Professional Science Editing for Scientists @ prosciediting.com