Bound material was sequentially eluted with 0.05 M Tris-HCl buffer and 2 M NaCl; a recovery of 0.27% by weight or 4 g total weight was achieved.
Powdered material was sequentially extracted with hexane (HX), benzene (BZ), chloroform (CH), ethyl acetate (EA), acetone (AC), ethyl alcohol (ET), and water (AQ) in Soxhlet apparatus as described earlier [ 19, 24].
Powdered material was sequentially extracted with hexane (HX), benzene (BZ), chloroform (CH), ethyl acetate (EA), acetone (AC), ethyl alcohol (ET), and water (AQ) in Soxhlet apparatus as described earlier [ 17, 18].
Powdered material was sequentially extracted with petroleum ether (PE), benzene (BZ), chloroform (CH), ethyl acetate (EA), acetone (AC), ethyl alcohol (ET), and water (AQ) in Soxhlet apparatus as described earlier [ 18, 19].
Briefly, ground material was sequentially extracted over a sintered glass funnel under vacuum with two volumes of 100 ml of ice cold 80% ethanol, 100% ethanol, chloroform:methanol (1 1) and 100% acetone.
For preliminary antibacterial activity test, 100 g of the powdered plant material was sequentially extracted with petroleum ether (least polar), chloroform, acetone and methanol (most polar) using maceration technique with continuous shaking (at 25°C for 72 hrs) using a shaker (GSL 400).
The chip and the surface of the slide confine a flow chamber, in which lanes of the immobilized biological material are sequentially exposed to sample.
In many cases the modules are connected and materials are sequentially processed in each module resulting a final, unique product or assembly.
Powdered plant materials were sequentially extracted with different solvents in a Soxhlet apparatus for 8 h according to the method described elsewhere [ 21].
Coarsely powdered plant material (rhizomes) was sequentially extracted [ 18] with acetone, ethanol and distilled water using the Soxhlet apparatus.
Insoluble material was washed sequentially with 95% ethanol, chloroform:methanol (1 1, v/v), and acetone.
