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All pooled samples from each depth were initially flooded with sterile PBS (IITD, Poland) in the volume ratio 1 : 1 and then with the sterile loop (10 μL of the diameter, Sterbios, Poland) the material was seeded in agar with 5% horse blood and other media.
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The materials were seeded with human osteoblast-like MG 63 cells (density from 8500 cells/cm2 to 25,000 cells/cm25,000
The surface of the C. albicans frozen aliquots was scraped with a loop and the material obtained was seeded on Sabouraud medium.
This material can be "seeded" with cells during extrusion and these cells will proliferate within the colloidal gel matrix.
Organ decellularization is emerging as a promising regenerative medicine approach as it is able to provide an acellular, three-dimensional biological scaffold material that can be seeded with living cells for organ reengineering.
Most of the tissue engineering strategies developed for the creation of hard tissue substitutes relies on the use of a temporary three dimensional (3-D) scaffold material in which cells are seeded and in vitro cultured prior to implantation.
In the first series of experiments described in Materials and methods, cells were seeded in transwell chambers and allowed to migrate across the filter or invade the Matrigel™ for 20 hours, either with or without D609 (50 μg/mL).
DFCs and dNC-PCs were seeded onto materials for indicated periods of time.
Since meteorites that slammed into the early Earth also encountered extreme heat conditions -- in the form of volcanoes and asteroid impacts -- the scientists say the early Earth might have been seeded with materials similar to those they found in the Sutter's Mill meteorite.
The cavities were filled with endodontic materials and, once set, cells were seeded on these surfaces and their morphology and number were analysed.
There are two major categories of cardiac tissue engineered materials: (1) the cardiac patch is seeded with MSCs to construct engineered heart tissue (EHT) and can be placed on the heart surface during surgery; (2) gel scaffolds made of gel materials can be used to encapsulate MSCs and be directly injected into the heart surface.
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