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A plate of as-cast NiAl bronze (NAB) material was sectioned from a large casting.
The material was sectioned with a glass knife using a Leica UC6 ultramicrotome (Leica).
After polymerization the material was sectioned with an Ultramicrotome S (Leica; Wetzlar, Germany).
The archival material was sectioned at four micron and placed on superfrost plus slides.
Material was sectioned with a Leica CM1900UV cryostat (Leica Microsystems, Wetzlar, Germany) to obtain 7 μm slices.
Formalin fixed and paraffin embedded material was sectioned, H&E stained an analyzed microscopically by a dedicated mouse pathologist (by JYC).
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Then, the length of rolled material is sectioned into two halves.
Any sections containing ABA materials were sectioned at 100 nm by use of a Leica ultracut UCT ultramicrotome, equipped with a 45° Diatome diamond knife (30-US, Electron Microscopy Sciences).
Finally, the materials were sectioned and stained with 1% toluidine blue before examination under an ECLIPSE 50i microscope (Nikon) (Hu et al. 2010).
The paraffin embedded biopsy materials were sectioned and stained with the Hematoxylin and eosin stain.
Total mastectomy materials were sectioned continuously from the nipple to the periphery at 5- to 7-mm intervals.
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