Sentence examples for material was resuspended from inspiring English sources

Ground material was resuspended in 1 ml of sterilized water and 10 μl drops of a dilution series were spotted onto PSA medium plate in triplicates.

The material was resuspended in 6 ml PBS and equally split into 3 Corex tubes.

The pelleted material was resuspended in PBS and the RNA isolated by extraction with phenol/chloroform followed by ethanol precipitation.

The resulting material was resuspended in DNase1 buffer with 40 Units of DNase 1 and 7 Units RNase A and incubated 15 min at room temperature.

Lyophilized material was resuspended at 20 mg/mL in 100% dimethyl sulfoxide (DMSO) and then diluted to required concentrations in 100% DMSO.

Alternatively, the pellet of ethanol insoluble material was resuspended in a large volume of 2× loading buffer (300 ul per 5×106 cells).

Samples of the original insoluble material, each of the washes, and the final sonicated material were resuspended in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) sample buffer for analysis by Western blotting.

The next morning, the culture was filtrated on Whatman paper (with a size cut-off of 15 µm) and 20 mg of wet material were resuspended in 100 ml of fresh LB, then treated with fresh formaldehyde (5% final concentration) for 30 min at RT followed by 30 min at 4°C.

For each condition, a total of 2.5 g of wet material were resuspended in 25 ml of lysis buffer consisting in 50 mM TRIS/HCl buffered at pH 8.0 at 20 °C and containing 0.1 M NaCl and Complete Mini protease inhibitor mixture (Roche Applied Science, one tablet/7 ml).

The TNA extraction was performed from 100 μl of EDTA blood, 200 μl of swab supernatant or vaccine material that was resuspended in 500 μl of PBS (see below) using the MagNa Pure LC (Roche Diagnostics AG, Rotkreuz, Switzerland) and the MagNa Pure LC TNA Isolation Kit (Roche Diagnostics) following the manufacturer's instructions.

Lysates were centrifuged for 10 min at 4°C, 13,000×g to separate insoluble material and cleared lysate was resuspended in 1× Laemmli sample buffer (Invitrogen, UK) under reducing conditions and heated for 10 min at 90°C.