The idea of a circular economy, in which a fixed quantity of material is repeatedly recycled, is a compelling one.
Samples were considered positive when >2 materials were repeatedly positive by 2 different PCRs.
After modification, the materials were repeatedly washed with chloroform, THF, methanol, and THF, before drying in a vacuum oven followed by further characterization.
Researchers have suggested that the manner by which some individuals share their NSSI experiences online may lead to reinforcement of the behaviour for some individuals when this e-material is repeatedly accessed [ 7, 9, 10, 12, 29, 30].
A part of each fixed soil suspension was boiled in 10%H2O22 solution to remove any organic material and was repeatedly rinsed with distilled water to obtain cleaned diatom frustules (Fujita and Ohtsuka, 2005).
To ensure thorough mixing of raw materials, the mixing was repeatedly done from one end of the spread polythene sheet to the other.
To obtain single layers of MoS2 in water suspension, the material was washed repeatedly with distilled water and centrifuged.
The fermented material was extracted repeatedly with EtOAc (4 × 1.0 L), and the organic solvent was evaporated to dryness under vacuum to afford the crude extract (50.0 g), which was fractionated by silica gel VLC using petroleum ether EtOAc MeOH gradient elution.
The material was washed repeatedly in 95% ethanol until the tissue was bleached.
The material was rinsed repeatedly in the same buffer and post-fixed for 2 h in 1% OsO4 in phosphate buffer, pH 7.4.
