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Briefly, a 100 g plant extract material was refluxed in 1000 ml of aqueous, methanol or chloroform and the respective mixtures were placed on an orbital shaker (GFL, model 3020, Germany) at 160 revolutions per minute (rpm) for 72 hours.
Similar(59)
The powdered material (500 g) was refluxed successively with the solvents petroleum ether, chloroform, and ethanol in a Soxhlet extractor for 48 h.
The reaction mixture was refluxed and stirred continuously under nitrogen until the starting material was completely exhausted.
The mixture was refluxed overnight.
The resultant solution was refluxed at 100 °C.
Reaction mixture was refluxed for 5 h.
The mixture was refluxed for 20-30 minutes.
The reaction was refluxed for 20 h.
This mixture was refluxed for 24 h.
The mixture was refluxed for 18 h.
The mixture was refluxed for 2 hours.
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