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The crude material was purified by flash chromatography to afford the product.
The starting material was purified by high performance liquid chromatography (HPLC) systems ranging from the analytical scale process to a scaleup to 1 g per batch.
The solution was concentrated and the crude material was purified by recrystallization.
The material was purified by microwave-induced oxidation of carbon nanoparticles and subsequent washing with a 37 wt.% HCl.
The crude material was purified by column chromatography (hexanes/ethyl acetate 2 1) to yield tritylated 10 (65 mg, 65%).
The crude material was purified by column chromatography (hexanes/ethyl acetate 3 1) to yield 9 (216 mg, 77%).
Raw nanotube material was purified in large batches, and the ropes were cut into 100- to 300-nanometer lengths.
The eluted material was purified with QIAquick PCR purification Kit, Qiagen.
Post-implant material was purified according to scheme reported in Figure 2a.
Solubilized His-tagged material was purified using Ni-affinity chromatography as previously published [21].
Refolded material was purified by RP-HPLC under conditions used for the linear form.
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