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A tissue slice obtained from the central and maximum cut surface of the surgical material was minced into small pieces.
Embryos were collected at 13 14 days gestation, the carcasses removed in PBS and the remaining material was minced then placed into 0.25% trypsin (Life Technologies Ltd. 15050-065) in Dulbecco's Modified Eagle Media with GlutaMAX (Life Technologies Ltd. 61965-026) then incubated at 37°C for 20 min with pipette-trituration.
Similar(58)
The dried plant material (9.4 g) of H. cuneiformis was minced and extracted exhaustively with methanol.
The crushed and dried plant material of Sargassum sp. (S. sect. Binderiana) (16 g) was minced and extracted exhaustively with methanol.
It doesn't feel like it was minced and reconstituted.
The brain tissue was minced and digested with trypsin.
The remainder of the embryo was minced.
Briefly, the tissue was minced and incubated in collagenase.
Cortical tissue was minced in ice-cold Krebs salt solution.
The tissue was minced and digested as above.
Isolated tissue was minced with a scalpel and/or forceps.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com