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The material was hydrolyzed with sulphuric acid in the presence of carboxylated multiwalled carbon nanotubes (MWCNTs) to promoted chemical interaction among cellulose and carbon surface.
Subsequently, the new imine-functionalized material was hydrolyzed in water (10 mL) containing acetic acid (0.25 μL) at 30°C for 1 h, and the regenerated imine concentration was determined by spectrophotometry at λ = 268.5 nm.
To estimate the glucan content, tobacco leaf material was hydrolyzed in sulfuric acid and the carbohydrates were determined using the anthrone assay [ 64].
When harsher treatment conditions were applied, more material was hydrolyzed to yield soluble compounds, due to an increased sugar polymer to monomer ratio at higher temperatures.
The pellets were weighed (values used for DM calculation), polymeric material was hydrolyzed with sulfuric acid, and neutral sugars were analyzed according to the method as described by van den Oever et al. (2003).
Approximately 1 3 mg of wall residue or fractionated material was hydrolyzed (2 M TFA, 110°C, 2 h) and the liberated monosaccharides converted to methoxy sugars using 1 M methanolic HCl at 80°C for 24 h.
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Grass and silage presscakes from various types of raw materials were hydrolyzed with dilute acid at moderate conditions to recover hemicellulose-derived sugars.
After pretreatment, the raw materials are hydrolyzed with Cellic CTec3 enzyme (Novozymes) for 24 hours.
In this work, we defined effective hydrolysis as at least 70% of the original cellulose in the pretreated lignocellulosic materials being hydrolyzed to glucose.
In this two-step approach, raw materials are hydrolyzed via a multienzyme pretreatment to convert the chemical oxygen demand (COD -containing COD -containing a soluble form.
The Arundo donax and spruce pretreated materials were hydrolyzed at an initial pH of 4.8, using enzyme complex ACCELLERASE® 1500 for 48 h at 53°C according to the protocol from the manufacturer.
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