The dried plant material was ground using a conventional rotor mixer.
The parent material was ground and sieved to choose the particle size within the range of 100 150 μm.
Before research, the raw material was ground to 2 5 mm and stored in desiccator for maintenance of constant humidity and chemical composition.
The dried material was ground into powder and starch content was determined at 630 nm with a spectrophotometer (UV2800PC, Hengping, Beijng).
For RNA isolations, infected leaf material was ground to a fine powder in liquid nitrogen.
Freeze-dried material was ground in 3 ml acetone/water/acetic acid (80/19/1; v/v/v), then centrifuged (4630 g, 4°C, 3 min).
Up to 30 mg of dry material was ground for 2 min in a TissueLyser mixer-mill disruptor (Qiagen, California, USA) using tungsten beads.
Leaf material was ground to a fine powder in liquid N2, and total RNA was isolated using the TRIzol Reagent (Invitrogen) according to the manufacturerś protocol.
Hypocotyl material was ground and clamped against the diamond element.
Fresh material was ground in 300 μl 1% methanol-HCl and incubated overnight at room temperature.
The raw material was ground and sifted with a 20 mesh screen.
