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Excess adhesive material was gently removed from around the bracket base without disturbing it.
Prior to analysis, parchment material was gently scraped by means of a file and the resulting powder was mixed with KBr (at approx. 1 30 w/w ratio) and pressed to form a KBr pellet.
The living stromatolite material was gently broken down by hand into constituent grains and passed through a 1 mm sieve to remove large fragments but retain the carbonate ooid grains and associated microbial populations.
The material was gently spread on the second slide by pulling the two slides facing each other gently apart.
To determine the density of the powdered blends, the material was gently poured into a 10 cm graduate cylinder, with bulk density (BD) being calculated as the ratio between the weight (g) and volume (cm).
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Ultimately, the material is gently lifted at one tip, and peeled off the skin.
'Sapric' peat consists for less than one-sixth of recognizable plant tissue after the material is gently rubbed.
For the adherence assay, after the incubation time, the materials were gently washed with sterile phosphate buffered saline (PBS) in order to remove the non-adherent bacteria and placed in 2 mL centrifuge tubes containing 1 ml of sterile PBS.
Sixty grams (60 g) of ground plant material was boiled gently with 1.6 L distilled water for approximately 3 h to reduce the volume to 200 mL.
The material was filtered through sterile gauze, and the filtrate was gently centrifuged for 3 min at 50 g.
The punctured cecum was gently squeezed to expel a 1- to 2-mm droplet of fecal material and returned to the abdominal cavity.
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