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Chimney material was frozen at −80°C immediately after collection and remained frozen until onshore analysis.
Plant material was frozen in liquid nitrogen.
The material was frozen and stored at -80°C.
For each samples, 2 g of material was frozen in liquid nitrogen and ground to powder.
No material was frozen until vitrification of the ribosomes on carbon coated holey cryogrids.
The remaining ∼25 µl of bisulfite converted DNA material was frozen at −80°C and stored.
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Below a certain temperature, known as the glass transition temperature (Tg), the molecules of a polymer material are frozen in what is known as the glassy state; there is little or no movement of molecules past one another, and the material is stiff and even brittle.
C-V curves measured at 77 K (Fig. 3b) show much lower hysteresis than those obtained at room temperature, thus indicating that some traps in the material are frozen at low temperatures [16].
Samples of fresh material were frozen at -22°C for later analysis.
All the materials were frozen in liquid nitrogen quickly after they were taken from the plant and then stored at –80 °C until use.
All materials were frozen in liquid nitrogen immediately after collection for ribonucleic acid (RNA) isolation.
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