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The gas and particulate phase material was fractionated by HPLC into nonpolar, moderately polar and highly polar chemical fractions.
The ammonium sulphate precipitated material was fractionated by ion-exchange (a); and the most active peaks were separated by gel filtration (b); the main active peak was further analyzed for purity by HPLC (c).
Cells were lysed and soluble material was fractionated by centrifugation through sucrose gradients.
The bound material was fractionated by SDS-PAGE and analyzed by IB with anti-puromycin antibody (top panel).
One fifth of the amplified product (corresponding to 2% of the original material) was fractionated on 6% polyacrylamide gels in Tris-borate-EDTA.
Samples were spun for 15 min at 20,000 x g to remove insoluble material, and the solubilized material was fractionated by gravity flow over a 1 ml Sephacryl-S-300 column pre-equilibrated in column buffer (50 mM HEPES [pH 7.5], 200 mM KOAc, 15 mM MgOAc2, 1 mM DTT, and 0.25% digitonin).
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In biomass refinery, lignocellulosic material is fractionated to obtain the main components (cellulose, hemicelluloses, and lignin).
For the purification of the three major polar 18 3-Glu derivatives, a leaf extract derived from wounded 18 3-Glu-treatederivativesata leafextractfractionatederivedLC as described in Materials and Methods.
The crystals were dissolved, and the DNA material, 5′-labelled by incubation with a polynucleotide kinase and γ-P-ATP, was fractionated through denaturing polyacrylamide gels.
Eluted material from R6/2 mice aged 2, 4, 6 and 12 weeks of age was fractionated by SDS PAGE alongside age-matched tissue lysates and immunoprobed with a series of antibodies: S830, MW1, MW8 and 3B5H10 (Fig. 6).
Insoluble material was removed by centrifugation at 16,000×g for 20 min. The supernatant, containing 1.5 mg of dissolved protein, was fractionated by HPLC gel filtration through a Superose 12 10/300 GL column (GE Healthcare).
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