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For elemental analysis, the dry plant material was finely ground.
The air dried plant material was finely powdered and stored at 4 °C.
Leaf material was finely ground using a ball mill (Kleco pulverizer).
The tumour material was finely chopped and placed in 20 ml of collagenase (1 mg ml−1) in a shaking water bath at 37°C for 20 min.
Air-dried plant material was finely crushed, powdered and successively extracted in polar to non-polar solvent on an increasing degree of non-polarity.
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Metallic plutonium is a fire hazard, especially if the material is finely divided.
All materials were finely ground using a stainless steel ball mill prior to analysis.
The starting materials were finely ground, inserted into a gold capsule, and placed in a boron nitride crucible (Henze BNP GmbH, HeBoSint® S100, Kempten, Germany).
The dried plant material was then finely ground and analyzed for N using a N-protein analyzer (Na2000, Carlo Erba, Milan, Italy).
The pod material was ground finely and transferred to a disposable 50 mL centrifuge tube containing 15 mL of 65°C extraction buffer [ 84].
Mixing four samples of each lot in a large bowl by hand produced a composite material, of which approximately 1 kg was finely ground to between 100 and 200 microns using a centrifugal mill (Retsch Mill ZM 200, Haan, Germany).
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