For the extraction procedure, 4 g of homogenized dry plant material was extracted by pressurized liquid extraction using an "ASE200" instrument (Dionex, Sunnyvale, CA, USA).
To estimate the levels of endogenous MeJA, plant material was extracted by solid phase micro-extraction without the derivatization step.
25 g of ground plant material was extracted successively with distilled H2O in Soxhelt extraction apparatus.
The plant material was extracted using crude alcohol (70%) by maceration and Reflux extraction after optimization.
Genetic material was extracted and analyzed with a machine from 454 Life Sciences, a company immersed in the race to make gene sequencing a fast, cheap technology.
The material was extracted by traction after 6 months.
Approximately 10 g of pulverized plant material was extracted with 100 mL solvent.
The plant material was extracted with 0.1 M HCl (3 L) by stirring with a magnetic stirrer for 90 min.
The powdered plant material was extracted in three different solvent (methanol, ethanol, acetone) for 12 h with the help of Soxlet apparatus.
A 50 g of the plant material was extracted with 200 mL of methanol using soxhlet apparatus and the resulting extract was concentrated using rotary evaporator.
A sample of 5.0 g of each plant material was extracted by 150 mL n-hexane using a Soxhlet apparatus until totally depleted.
