Sentence examples for material was eluted from inspiring English sources

The column was then washed with PBS and the bound material was eluted with elution buffer containing 10 mM Glutathione in 50 mM Tris-HCl, pH8.0.

The immunoprecipitated material was eluted in elution buffer (100 mM NaHCO3, 1% SDS) and was then digested with proteinase K and RNase H to remove protein and RNA, respectively.

After washing, the supernatant was removed and total material was eluted with 100 µl elution buffer (0.1% SDS, 2.5 mM Tris pH 6.8 and 5 mM DTT) at room temperature.

Finally, the remaining material was eluted with 1 wt% DOC, which resulted in complete elution.

ChIPed material was eluted by two 15 min incubations at RT with 225 μl elution buffer (0.1 M NaHCO3, 1% SDS).

Bound material was eluted in 1 ml of 0.5%Tween-20Tween-20, bacteria in the elution fraction were collected in a minifuge at 5220 g for 5 min, resuspended in deionized water and 100 μl portions were spread onto BHI/kanamycin agar plates.

The extract is passed through an ion exchange column to absorb the anthocyanin material and the adsorbed material is eluted by means of acetone, alkali or dimethyl formamide (DMF).

Precipitated materials were eluted with 300 μl elution buffer (1% SDS; 0.1 M NaHCO3; 50 mM Tris HCl, pH 8; and 10 mM EDTA).

After washed with ChIP wash buffer (50 mM HEPES-KOH (pH 7.0), 0.5 M LiCl, 1 mM EDTA, 0.7% deoxycholate, 1% NP-40) five times and with TE (10 mM Tris-HCl (pH 7.5), 1 mM EDTA) once, immunoprecipitated materials were eluted from the beads by heating overnight at 65 °C in elution buffer (50 mM Tris-HCl (pH 8.0), 10 mM EDTA, 1% SDS).

After extensive washing, bound materials were eluted with Laemmli buffer and analysed by immunoblotting.

At this stage, unreacted monomers and other low molecular weight materials are eluted along with low-affinity polymer nanoparticles.