Schreier (2014) suggests that at least some parts of the material be double-coded for the sake of clarity and unambiguity, and in order to test data coding quality.
The process productivity is doubled and material pickup, especially problematic in the output channel, avoided.
Subtracted libraries were also produced; for two libraries (11 and 12), cDNA for alpha-lactalbumin, beta-lactoglobulin, alpha-casein, beta-casein and kappa-casein was removed from the cDNA pool following the manufacturers instructions for the SMART subtraction library (Promega) except that the subtracted material used was double stranded cDNA corresponding to the five genes listed above.
The filter material was wetted with double distilled autoclaved water prior to filtering the E. coli bacterial suspension.
This material was synthesized with double ligands (bdc and ted) by a hydrothermal method.
After filtration, the obtained solid material was grinded with double amount of melanin and sintered under Ar atmosphere for 4 h at 750°C.
In the few cases in which available material was insufficient for double coverage, the sequence result is regarded as tentative.
150.3 g dried, ground material was extracted using double distilled water (2 L) and boiled for one hour.
The material was passed through a double layered muslin cloth and the fluid was subsequently filtered through a Watman-1 filter paper.
The fresh and disease free plant material was washed twice with double distilled water, shade dried at room temperature for 21 days and then grinded to powder.
