Open and pre-anthetic flowers of this material were dissected, observed, and photographed under a Leica EZ4D stereomicroscope (Leica Microsystems).
The necrotic tissue and abscess materials were dissected under stereotactic microscopy with a Beaver 66 surgical blade and scissors, until the choroidal pigmentation was visible through the scleral fibers.
According to previous reports [6 8], the size of about 2,500,000 and 1,000,000 μm2 of each separated tissues of fresh and dried materials were dissected by LMD respectively which could detect the chemicals containing in tissues.
For scanning electron microscopy, fixed materials were dissected, dehydrated in ethanol and critical point dried in a Denton critical point dryer.
Briefly, materials were dissected under a stereomicroscope and treated with 0.002 mol/L 8-hydroxyquinoline for 3 h in total darkness at room temperature, followed by fixation Carnoy's fixation solution (ethanol: acetic acid = 3: 1, v/v) for at least 1 h at room temperature.
Material for each tissue homogenate was dissected from the center of each tissue sample by using single-use disposable instruments and rigorous sterile procedures.
To exclude the effect of Ezh2 deletion on erythropoiesis, the liver primordium was dissected out (supplementary material Fig. S5A) before disassociation of embryos.
The fundal myoma was dissected from the uterus, resulting in an outflow of purulent fluid and colliquative necrotic material.
