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Second, for seeding, this material was diluted 10-fold that corresponds to the limiting dilution of atypical PrPres (Fig. 1c) (dgPMCAb titration of atypical PrPres material is in Additional file 1: Figure S1).
Freeze-dried material was diluted in deionized water, followed by addition of CuCl2.
To investigate the appearance of the PS-NPs in soil, the soil material was diluted to a concentration of 5 mg mL−1 by water (HiPerSolv CHROMANORM® for HPLC).
The material was diluted or converted to a form that can't readily be enriched to a higher level.
The B-PER extracted material was diluted four-fold with water, and applied to a Q-Sepharose column.
The insoluble fraction was removed by ultracentrifugation (20 min 267,008×g at 4°C), after which the solubilized material was diluted five-fold in buffer A, supplemented with 200 mM KCl and 15 mM imidazole pH 8.0 to reduce the detergent concentration.
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Total activity potency (TAP) is the volume at which the components, extracted from 1 g of material, are diluted enough to maintain the same potency against the test pathogens.
During Biacore studies, the chips were submitted to continuous flow of HBS-P buffer (10 mM Hepes, 0.15 M NaCl, and 0.05% P20 detergent, pH 7.4), and all injected material were diluted in the same buffer.
An important piece of the puzzle of how hereditary material is diluted at each generation has been elucidated over the past ten years.
In contrast, using the solids washing option, the pretreated material is diluted with recycled process water to a pumpable level and fed to the solid liquid separation equipment.
For the Repli-G reactions, 25 ng of genomic material were diluted in TE to a final volume of 2.5 μl.
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