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The labeled material was denatured at 95°C for 3 minutes and cooled to 42°C before injection in the hybridization chamber.
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After printing, the material on the slides was denatured by incubation in hot water (95°C) for 2 min, then dried by centrifugation.
Total RNA was denatured and blotted onto nitrocellulose membranes.
DNA was denatured and processed by standard Southern blot procedure.
To prevent AE1 aggregation, the blood was denatured to 65°C for 10 minutes, while the heart was denatured at 95°C.
The sample was denatured at 94°C.
This part of the β-casein was denatured only slightly.
Genomic DNA was denatured for 2 minutes at 84°C.
Collagen was denatured by heating for 45 minutes at 70°C.
Then, the enzyme was denatured at 98°C for 15 minutes.
The mixture was denatured at 85 °C as before.
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